- Isolate Module
- Isolate list
- Register isolate
- Antigenic Module
- Run list
- Run set up
- Run data entry
- Quality control
- Antigenic mapping (displaying an antigenic map of a run)
- Antigenic and genetic comparison
- Genetic Module
- Sequence list
- Upload sequence
- Phylogenetic Module
- Collection list
- Add new sequence
- Collection, alignment, and Tree creation
- Project Manager Module
- Reference kit
- Reference Antigen
- Reference Antisera
- Summary report
- Members of IVM Online routinely accept and process all AI samples from the field and only H5 that have been through virus isolation process will go into the next phase.
- Those allantoic H5 HPAI Virus is identified by using hyper-immune sera like NDV and H5.
- Positive H5 HPAI viruses are then PRE-SCREENED using the PRE-SCREEN KIT containing 3 antigens, 3-4 prime sera and H5 hyper-immune antiserum. The data of the HI titres along with epidemiological data of every isolate are then uploaded to IVM Online.
- This pre-screen data is then analyzed by the FOCAL POINT (DIC WATES) by using an analysis module in IVM Online to evaluate the selected isolate. All members have been requested to submit their selected isolates to DIC WATES to be propagated, and antigenically characterised using a pre-screen and a FULL PANEL KIT containing 7-8 antigens and antisera (prime and hyper-immune). The HI titres from the full panel antigenic characterization are uploaded to IVM Online and re-analyzed.
- Identified variant strains are then sequenced by sequencing partners (DIC BUKITTINGGI, PUSVETMA, BBLITVET, and BBPMSOH). The results of the HA gene sequencing in the form of a fasta file is uploaded to IVM Online.
- Bioinformatics analysis of the sequencing and antigenic characterization results is conducted and mapped by the FOCAL POINT, and reported to the Management Team.
- If the management team finds the presence of ANTIGENIC SHIFT in variant strains, then the management team reviews the data completely, including epidemiological and phylogenetic tree data of the variant strain; if necessary, the management team will immediately recommend changing the challenge strain seed to the Director of Animal Health.
- IVM Online activities conducted by competent officers/staff.
- IVM Online staff/officers’ competence is developed through Competency Based Training.
- Training is determined and performed both internally by focal points and externally.
- For QUALITY ASSURANCE and QUALITY CONTROL, the implementation of IVM Online activity follows TECHNICAL GUIDELINES and the STANDARD OPERATIONAL PROCEDURES – SOP
- Meetings between IVM Online members are held every year to ensure the continuation of the IVM Online system.